Journal of Pharmaceutical Care 2014. 2(4):162-169.

The Investigation of the Antioxidative Properties of the Synthetic Organoselenium Compounds in Liver Tissue of Rat with Histological and Biochemical Analyses
Zeliha Selamoglu, Ismet Yilmaz


Background: Oxidative stress is described as the formation of toxic effect due to the deficiency of cellular antioxidative level toward the level of reactive oxygen species (ROS). The excess production of ROS or the decrease in the antioxidative defense system could be the cause for oxidative stress. 7,12-dimethylbenz[a]anthracene (DMBA) that is known to be the major cause the increment in lipid peroxidation level and the oxidative damage in the rat liver. As a fundamental trace elements, selenium as a part of anti-oxidative defense system is responsible for the immune system as part of enzymes in defense system.
Methods: Organoselenium compounds [1-isopropyl-3- methylbenzimidazole-2-selenone (Se I ) and 1, 3-di-pmethoxybenzylpyrimidine- 2-selenone (Se II)] that were prepared in the laboratories. The effects of synthetic organoselenium compounds (Se I and Se II) against DMBA-induced changes in levels of some [catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GR) activities and total glutathione (GSH), malonedialdehyde (MDA)] parameters in rat liver were investigated. Histopathological changes in the liver tissues of rats were examined by light microscopy.
Results: Because the selenium has an anti-oxidative properties toward the damaged induced cells, organoselenium compounds prepared in our laboratories, Se I and Se II, have tested for chemically induced rat liver tissues. The results showed that endogen antioxidant enzymatic activities changes and the preventing of oxidative damage in lipid peroxidation are important findings in vivo of this research.
Conclusion: Various changes were observed in liver tissue of rats in the all experimental groups.


7;12-Dimethylbenzanthracene; Histology; Liver; Oxidative Stress; Organoselenium Compounds

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Das RK, Ghosh S, Sengupta A, Das S, Bhattacharya S. Inhibition of DMBA/ croton oil-induced two-stage mouse skin carcinogenesis by selenocyanate. Eur J Cancer Prev 2004; 13(5): 411-7.

Demir I, Demirbag Z. Biodegradation of polycyclic aromatic hydrocarbons. Tr J Biol 1999; 23: 293-302.

Talas ZS, Ozdemir I, Yilmaz I, Gok Y, Orun I. The investigation of the antioxidative properties of the novel synthetic organoselenium compounds in some rat tissues. Exp Biol Med 2008; 233: 575-9.

Ghosh P, Singha Roy S, Basu A, Bhattacharjee A, Bhattacharya S. Sensitization of cisplatin therapy by a naphthalimide based organoselenium compound through modulation of antioxidant enzymes and p53 mediated apoptosis. Free Radic Res 2015; 49(4):1-19.

Singha Roy S, Chakraborty P, Ghosh P, Ghosh S, Biswas J, Bhattacharya S. Influence of novel naphthalimide-based organoselenium on genotoxicity induced by an alkylating agent: the role of reactive oxygen species and selenoenzymes. Redox Rep 2012;17(4):157-66.

Pagmantidis V, Meplan C, van Schothorst EM, Keijer J, Hesketh JE. Supplementation of healthy volunteers with nutritionally relevant amounts of selenium increases the expression of lymphocyte protein biosynthesis genes. Am J Clin Nutr 2008; 87: 181-9.

El-Bayoumy K, Sinha R. Mechanisms of mammary cancer chemopreventionby organoselenium compounds. Mutat Res 2004; 551:181-97.

Garud DR, Koketsu M, Hideharu I. Isoselenocyanates: a powerful tool for the synthesis of selenium-containing heterocycles. Molecules 2007; 12: 504-35.

Sieja K, Talerczyk M. Selenium as an element in the treatment of ovarian cancer in women receiving chemotherapy. Gynecol Oncol 2004; 93: 320-7.

Talas ZS, Ozdemir I, Gok Y, Ates B, Yilmaz I. Role of selenium compounds on tyrosine hydroxylase activity, adrenomedullin and total RNA levels in hearts of rats. Regul Pept 2010; 159: 137-41.

Talas ZS, Bayraktar N, Ozdemir I, Gok Y, Yilmaz I. The effects of synthetic organoselenium compounds on nitric oxide levels in DMBA- induced rat liver. J Environ Biol 2009; 30(4): 591-3.

Laskin JD, Heck DE, Gardner CR, Laskin DL. Prooxidant and antioxidant functions of nitric oxide in liver toxicity. Biotechnology 2001; 3: 261-71.

Nagar S, Zalator JJ, Blanchard RL. Human UGT1A6 pharmacogenetics: Identification of a novel SNP, characterization of allele frequencies and functional analysis of recombinant allozymes in human liver tissue and in cultured cells. Pharmacogenetics 2004; 14: 487-99.

Talas ZS, Gok Y, Ozdemir I, Ates B, Gunal S, Yilmaz I. Synthesis, antioxidant and antimicrobial properties of two organoselenium compounds. Pak J Pharm Sci 2015; 28(2): 611-16.

Washington DC. Guide for the care and use of laboratory animals. National Academy Press, 1996.

Lowry O, Rosebrough NJ, Farr AL, Randall RJ. Protein measurements with the folin phenol reagent. J Biol Chem 1951; 193:265–75.

Luck H. Methods of Enzymatic Analysis. Verlag Chemie, Academic Press, New York, (1963) pp.885–8.

McCord JM, Fridovich I. Superoxide dismutase: an enzymatic function for erythrocuprein (hemocuprein). J Biol Chem 1969; 244: 6049-55.

Lawrance RA, Burk RF. Glutathione peroxidase activity in seleniumdeficient rat liver. Biochem Biophys Res Commun 1976; 71: 952-8.

Carlberg I, Mannervik B. Glutathione reductase. Methods Enzymol 1985; 11: 484-490.

Theodorus P, Akerboom M, Sies H. Assay of glutathione, glutathione disulfide and glutathione mixed disulfides in biological samples. Methods Enzymol 1981; 77: 373-83.

Beuge JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 1978; 52: 302-10.

El-Demerdash FM. Antioxidant effect of vitamin E and selenium on lipid peroxidation, enzyme activities and biochemical parameters in rats exposed to aluminium. J Trace Elem Med Biol 2004; 18: 113-21.

Shadab GG, Ahmad ME, Azfer MA. Anticlastogenic action of vitamin C against genotoxicity of estrogenic drug diethylstilbestrol (DES) in the human lymphocyte chromosomes. J Environ Biol 2006; 27: 85-88.

Ozdemir I, Talas ZS, Gok Y, Ates B, Yilmaz I. Changes in tyrosine hydroxylase activity, adrenomedullin and total RNA levels by treatment of organoselenium compounds in rat hypothalamus exposed to DMBA. Fresen Environ Bull 2010; 19(4a): 664-8.

Talas ZS, Ozdemir I, Yilmaz I, Gok Y. Antioxidative effects of novel synthetic organoselenium compound in rat lung and kidney. Ecotox Environ Safe 2009; 72(3): 916-21.

Talas ZS, Yilmaz I, Ozdemir I, Ates B, Gok Y, Cetinkaya B. Role of synthesised organoselenium compounds on protection of rat erythrocytes from DMBA-induced oxidative stress. Biol Trace Elem Res 2009; 128: 167- 75.

Ozdemir I, Talas ZS, Gul M, et al. Inhibition of DMBA induced rat mammary duct damage by novel synthetic organoselenium compounds. Exp Anim 2006; 55(5): 449-55.

Selamoglu Z, Ozdemir I, Ates B, Gok Y, Orun I, Yilmaz I. The effects of novel synthetic organoselenium compounds on hematological parameters in DMBA-induced rats. Fresen Environ Bull 2007; 16(6): 596-600.

Ozdemir I, Selamoglu Z, Ates B, Gok Y, Yilmaz I. Modulation of DMBAinduced biochemical changes by organoselenium compounds in blood of rats. Indian J Biochem Biophys 2007; 44(4): 257-9.

Rayman MP. The importance of selenium to human health. Lancet 2000; 356: 233-41.

El Bayoumy K. The protective role of selenium on genetic damage and on cancer. Mutat Res 2001; 475: 123-39.

Duffield-Lillico AJ, Reid ME, Turnbull BW,et al. Baseline characteristics and the effect of selenium supplementation on cancer incidence in a randomized clinical trial: a summary report of the nutritional prevention of cancer trial. Cancer Epid Biomarkers Prev 2000; 11: 630-9.

Basu A, Bhattacharjee A, Singha Roy S, et al. Vanadium as a chemoprotectant: effect of vanadium(III)-L-cysteine complex against cyclophosphamide-induced hepatotoxicity and genotoxicity in Swiss albino mice. Eur J Biochem 2014; 19(6): 1141-6.

Bhattacharjee A, Basu A, Ghosh P, Biswas J, Bhattacharya S. Protective effect of Selenium nanoparticle against cyclophosphamide induced hepatotoxicity and genotoxicity in Swiss albino mice. J Biomater Appl 2014; 29(2): 303-17.

Carlo WF, Hummer AJ, Schwartz L, et al. Extent of hepatic resection does not correlate with toxicity following adjuvant chemotherapy. J Surg Oncol 2004; 87: 85-90.


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