Clinical Effects of Chlorhexidine 0.2% and Cetylpyridinium 0.05% Combination in Comparison with Chlorhexidine, Cetylpyridinium and Persica in Reducing Oral Bacteria in Healthy Individuals

  • Nioosha Yarahmadi Department of Clinical Pharmacy, Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
  • Farshad Hashemian Mail Department of Clinical Pharmacy, Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
  • Reza Hosseini Doust 2Department of Microbiology, Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Chlorhexidine, Cetylpyridinium, Persica, Gingivitis, Periodontitis, Oral Bacteria


Background:  Preparation of a new product with the goal of reducing chlorhexidine’s side effects without decreasing (and even increasing) its effectiveness is a desirable goal for researchers in the field of oral hygiene. The aim of this study was to evaluate the efficacy of Chlorhexidine 0.2% and Cetylpyridinium 0.05% combination in reducing oral bacteria in comparison with Chlorhexidine 0.2%, Cetylpyridinium 0.05% and Persica mouthwashes.
Methods: 100 healthy volunteers aged between 18 and 30 years were randomly assigned to 5 groups. The first group received Chlorhexidine 0.2%, the second group received Cetylpyridinium 0.05%, the third received Persica, the fourth received Chlorhexidine 0.2% plus Cetylpyridinium 0.05%, and the fifth group received Chlorhexidine 0.05% plus Cetylpyridinium 0.05%. Samples were obtained at baseline and thirty minutes after oral rinsing with the mouthwashes. The number of colony-forming units (CFU/mL) before and after mouthwash administration was compared for each sample.  
Results: The preparation with the most bacterial count reduction was found to be Chlorhexidine 0.2% and Cetylpyridinium 0.05% combination. However, the difference between efficacy of Chlorhexidine 0.2% plus Cetylpyridinium 0.05% and Chlorhexidine 0.05% plus Cetylpyridinium 0.05% was found not to be statistically significant.                                                       

Conclusions: A new mouthwash preparation including chlorhexidine 0.05% and cetylpyridinium 0.05 % combination is the most desirable due to the increased efficacy and fewer side effects.


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How to Cite
Yarahmadi N, Hashemian F, Hosseini Doust R. Clinical Effects of Chlorhexidine 0.2% and Cetylpyridinium 0.05% Combination in Comparison with Chlorhexidine, Cetylpyridinium and Persica in Reducing Oral Bacteria in Healthy Individuals. J Pharm Care. 8(3):116-122.
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