Sensitivity of Acinetobacter baumannii and Pseudomonas aeruginosa Microorganisms to Colistin Antibiotic by MIC (E-test) in Patients Admitted to the Intensive Care Unit of Firoozgar Hospital
Background: Nosocomial infections are associated with increased morbidity, mortality, and medical burdens. Pseudomonas aeruginosa and Acinetobacter baumannii are not-fermentative gram-negative bacteria that considered as the most important nosocomial infection. In the current study, we have aimed to evaluate the sensitivity of Acinetobacter baumannii and Pseudomonas aeruginosa microorganisms to the colistin antibiotic.
Methods: In this descriptive cross-sectional study, patients admitted to the ICU ward of Firoozgar Hospital from July 2018 to March 2019 were evaluated, and 169 Patients infected with Acinetobacter baumannii, and Pseudomonas aeruginosa were included. Acinetobacter baumannii and Pseudomonas aeruginosa were isolated, and antibiotic sensitivity was determined by the disk diffusion method according to Clinical & Laboratory Standards Institute (CLSI) criteria. E test was also used to determine MIC-50 and MIC-90 of colistin.
Results: Acinetobacter baumannii was around 8 times more frequent than Pseudomonas aeruginosa. Colistin resistance was detected in only 4(2.4%). The mean age of patients infected by Acinetobacter baumannii was significantly higher than those infected with Pseudomonas aeruginosa. Moreover, the mean time of the hospitalization period did not show any significant differences in the different groups.
Conclusion: Our findings indicated that the majority of isolated Pseudomonas aeruginosa and Acinetobacter baumannii were sensitive to Colistin. Therefore, it could be effectively used for patients with a confirmed diagnosis of Pseudomonas aeruginosa and Acinetobacter baumannii.
2. Cerceo E, Deitelzweig SB, Sherman BM, Amin AN. Multidrug-Resistant Gram-Negative Bacterial Infections in the Hospital Setting: Overview, Implications for Clinical Practice, and Emerging Treatment Options. Microb Drug Resist 2016;22(5):412-31.
3. Livermore DM, Hope R, Brick G, Lillie M, Reynolds R. Non-susceptibility trends among Pseudomonas aeruginosa and other non-fermentative Gram-negative bacteria from bacteraemias in the UK and Ireland, 2001-06. J Antimicrob Chemother 2008;62 (Suppl 2):ii55-ii63.
4. Matar GM. Editorial: Pseudomonas and Acinetobacter: From Drug Resistance to Pathogenesis. Front Cell Infect Microbiol 2018;8:68.
5. Armin S, Karimi A, Fallah F, et al. Antimicrobial resistance patterns of Acinetobacter baumannii, Pseudomonas aeruginosa and Staphylococcus aureus isolated from patients with nosocomial infections admitted to Tehran hospitals. Archives of Pediatric Infectious Diseases 2015;3(4):e32554.
6. Cai B, Echols R, Magee G, et al. Prevalence of Carbapenem-Resistant Gram-Negative Infections in the United States Predominated by Acinetobacter baumannii and Pseudomonas aeruginosa. Open Forum Infect Dis 2017;4(3):ofx176.
7. Blair JMA, Webber MA, Baylay AJ, Ogbolu DO, Piddock LJV. Molecular mechanisms of antibiotic resistance. Nat Rev Microbiol 2015;13(1):42-5.
8. Nation RL, Li J. Colistin in the 21st century. Curr Opin Infect Dis 2009;22(6):535-43.
9. Gupta S, Govil D, Kakar P, et al. Colistin and polymyxin B: A re-emergence. Indian J Crit Care Med 2009;13(2):49-53.
10. Sadeghfard NK, Ranjbar R, Ghasemi A, et al. A study of antimicrobial resistance of acinetobacter baumannii and non-acinetobacter baumannii strains isolated from three hospitals in Tehran. Journal of Ilam University of Medical Sciences 2006;14:29-34.
11. De Francesco MA, Ravizzola G, Peroni L, Bonfanti C, Manca N. Prevalence of multidrug-resistant Acinetobacter baumannii and Pseudomonas aeruginosa in an Italian hospital. J Infect Public Health 2013;6(3):179-185
12. Boustanshenas M, Defaee S., Majidpour A, et al. Reliability of MIC Gradient Strips (E-test) in Detection of Colistin Resistant Acinetobacter baumannii Caused an Outbreak in a Teaching Hospital in Tehran. Infection Epidemiology and Microbiology 2018;4(3):99-103.
13. Huang H, Chen B, Liu G, et al. A multi-center study on the risk factors of infection caused by multi-drug resistant Acinetobacter baumannii. BMC Infect Dis 2018;18(1):11.
14. Sengstock DM, Thyagarajan R, Apalara J, Mira A, Chopra T, Kaye KS. Multidrug‐Resistant Acinetobacter baumannii: An Emerging Pathogen among Older Adults in Community Hospitals and Nursing Homes. Clin Infect Dis 2010;50(12):1611-6.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.